In tube 1 Azide – 0.04M stock, 0.5mL, DCIP 0.5mM stock, 0.5 mL, Malonate 0.2M stock, 0.2 mL, Succinate 0.02M stock, 0.5 mL and Mitchondrial suspension and 1mL of homogenization buffer to give a final reaction volume of 3 mL. How do I figure out the dilution factor (equation)?

How do you calculate the amount of protein in this assay.

would i use this formula?

To calculate the substrate concentration, use the following formula:


(stock conc.)(volume) = (assay conc.)(total assay volume).

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